Montana INBRE Infrastructure Resources


 

Functional Genomics Core Facility

 

MSU-Bozeman
Cooley Hall, Room 308
Phone: (406) 994-5666


Funded by the MT INBRE, the National Science Foundation, and the MJ Murdock Charitable Trust, the Functional Genomics Core Facility provides instrumentation and support for academic investigators throughout Montana and the Rocky Mountain west. 

 

The Core's current instrumentation and software includes: 

 

(1) Agilent Bioanalyzer for qualitative assessment of RNA integrity

(2) Affymetrix GeneChip station:  Hyb Oven 640, Fluidics Station 450, G7 Scanner and GCOS v1.4 processing software

(3) Slide-type microarray station:  hyb oven with rotation; Tecan HS400 hybridization and fluidics station (accomodates four full length slide arrays); and GenePix 4000B dual wavelength scanner with GenePix Pro data extraction software

(4) qPCR station:  Corbett CAS-1200 liquid handling robot, five-laser RotorGene 3000 qPCR cycler, and five-laser RotorGene 6000 with high resolution melt for qPCR and SNP detection

(5) Analysis software including GeneSpring GX v7.3 and v9.0, MeV (open-source, for ANOVA and SAM-based analysis), VAMPIRE (open-source, for probability-based analysis) and REST-RG (open-source, for qPCR data analysis). 

(6) Zeiss-PALM laser-capture/catapult (UV) microdissection microscope and optical tweezers (IR) for manipulation of living or fixed cells and tissues.

 

For most instrumentation, the facility provides instruction and supervision followed by independent user access.  For those doing Affymetrix microarrays, the facility can also accept RNA samples and provide full-service processing. 

 

In addition to equipment access and experimental support, investigators are strongly encouraged to contact the facility for assistance and feedback during the experimental planning and grantmanship phases.  We are happy to review aims, grant proposals and manuscripts for appropriateness.

 

Kate McInnerney, Research Associate, manages the facility and provides training and support to users, under the direction of Drs. Jean Starkey and Michael Franklin, of the Department of Microbiology.   Kate can be reached at 406.994.5666 or by email:  kmcinnerney@montana.edu.